The SSP detected a minimum of 56.25% of low AP herds (AP ≤2.0%) up to a maximum of 100% of herds with a within-herd AP ≥8.0%. A highly significant association was shown between the median AP herd status (>5%) and positivity to at least one ILMF or BTM sample. A significant difference in within-herd AP was shown between SSP MAP-positive herds and SSP MAP-negative herds. There was a total of 126 SSP MAP-positive herds (i.e., 21.3% SSP MAP-positive herds 95% confidence interval = 18.0–24.9) the within-herd apparent prevalence (AP) ranged between 0.00 and 22.73% (mean 6.07%). Additionally, 12,016 individual milk samples were collected: 9,509 from 102 SSP-positive herds (SSP MAP-positive) and 2,507 from 21 randomly selected SSP-negative herds (SSP MAP-negative). Samples from BTM and ILMF were collected twice from 569 dairy herds in southern Italy. The objective of this study was to evaluate the sensitivity and specificity of a screening sampling plan (SSP) to detect MAP-positive dairy herds by repetitive analysis of bulk tank milk (BTM) samples by ELISA and in-line milk filter (ILMF) samples by PCR. Infected herds must be identified in order to implement programs designed to reduce the incidence of disease within and between herds and to prevent MAP from entering the food chain. Paratuberculosis represents a dual problem for the milk production chain: in addition to economic losses to affected herds, MAP may have zoonotic potential. paratuberculosis (MAP) is the etiological agent of paratuberculosis, a chronic contagious bacterial disease primarily affecting dairy cattle. It does not store any personal data.Mycobacterium avium ssp. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
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